Figure 3
From: Sorting nexin 12 interacts with BACE1 and regulates BACE1-mediated APP processing
SNX12 does not affect γ-secretase activity. HEK-APPSwe cells were transfected with (A) SNX12 and control plasmids, or (B) two SNX12 siRNAs (1 and 2) and a control scrambled siRNA. Two γ-secretase components, PS1-NTF and nicastrin (NCT), were analyzed by Western blot. (C) HEK 293 T cells were first transfected with Notch NΔE. After splitting equally, cells were transfected with SNX12 (+) or control (−) plasmids. Notch NΔE and NICD in cell lysates were analyzed by Western blot. (D) HEK 293 T cells were first transfected with APP βCTF/C99. After splitting equally, cells were transfected with SNX12 (+) or control (−) plasmids. Transfected C99 and SNX12 in cell lysates were analyzed by Western blot. Aβ40 and Aβ42 in conditioned media were assayed by ELISA. N = 4.