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Figure 7 | Molecular Neurodegeneration

Figure 7

From: LDLR-related protein 10 (LRP10) regulates amyloid precursor protein (APP) trafficking and processing: evidence for a role in Alzheimer’s disease

Figure 7

LRP10 overexpression alters APP processing in SH-SY5Y cells. APP cleavage products in SH-SY5Y stable clones expressing pcDNA3 vector alone (Ctl) or HA-tagged LRP10wt or LRP10 trafficking mutant LRP102DXXAA. (A) Representative Western blot of total sAPP secreted in the media and of β-CTF and total APP in the cell lysates of the indicated SH-SY5Y stable clones. Actin was used as loading control. (B) AlphaLISA quantitative analysis of sAPPα, sAPPβ, and Aβ40 in the media of the SH-SY5Y stable clones expressing pcDNA3 vector alone (Ctl), high level of HA-tagged LRP10wt (LRP10wt) or low and high levels of LRP10 trafficking mutant (LRP102DXXAA). Results are expressed as means ± SD (n = 3). *, p < 0.01; **, p < 0.005; ***, p < 0.001 (compared with control cells).

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