Primary hippocampal cultures transduced with rAAV1-PGRN promotes neurite outgrowth and branching. (A) Mouse Pgrn ELISA of endogenous Pgrn levels in hippocampal primary neuronal cultures. (B) Human PGRN ELISA shows increasing levels of hPGRN generated in the media of treated primary cultures. (C,D) Bar graph quantification of total outgrowth (C) and branching (D)of primary hippocampal neurons transduced with increasing amounts of rAAV1-PGRN in WT cultures normalized to WT rAAV1-GFP treated cultures. (E,F) Bar graph quantification of normalized total outgrowth (E) and branching (F)in Grn−/− hippocampal neurons transduced with rAAV1-PGRN normalized to WT rAAV1-GFP treated cultures. Note that rAAV1-GFP was supplemented in rAAV1-PGRN non-treated cultures. Data is presented as the mean ± SEM. *p < 0.05; ***p < 0.001.