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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Bone marrow-derived fibroblast growth factor-2 induces glial cell proliferation in the regenerating peripheral nervous system

Figure 3

FGF-2 secreted by BMMC increases SC migration from sciatic nerve explants. A-F: Crushed sciatic nerve explants from distal nerve stump kept for ten days in culture under specific experimental conditions. A: Phase contrast image showing one explant with cells migrating from the tissue. B-F: Epifluorescent microscopy of migrating SCs immunolabeled for S-100β (green) and incubated with control medium (A), BMMC-CM (B), BMMC-CM+ neutralizing FGF-2 (C), rhFGF-2 added to the control medium (D) or rhFGF-2 + neutralizing FGF-2 (E). Cell nuclei were counterstained with DAPI. F: Quantitative analysis of the number of S-100β migrated SCs from sciatic nerve explants after incubation under different experimental conditions. Statistics: p<0.0001 ANOVA. Non-Schwann cells are indicated by thick or thin arrows in B. Scale bar: A-F= 50 µm.

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Molecular Neurodegeneration

ISSN: 1750-1326