Figure 5

Increased expression of FGF-2 is time correlated with increased satellite cell proliferation in the DRG after nerve lesion and BMMC treatment. A-D; F-I: Longitudinal sections of dorsal root ganglia (DRG) of adult rats. A: Low magnification photomicrograph of a longitudinal section of an uninjured DRG stained with neutral red. Insert show neurons in different cell size (low, medium and big size). B-D: Optical sections of DRGs slices by confocal microscopy taken 10 days after total transection immunolabeled for FGF-2 (red) and counterstained with TO-PRO (blue) treated with PBS (C) or BMMC (D) or uninjured sciatic nerve (B). E: Quantification of the fluorescence intensity compared between the experimental groups cited above. Statistics: p<0,0001 ANOVA. F-I: Optical.