Figure 4

Monomeric α-syn preferably coaggregates with pTau oligomers in presence of metal ions. A. Cross-correlation amplitudes (G (0)) show mild coaggregation of α-syn monomers with pre-formed tau oligomers induced by DMSO and Fe³⁺, while Al³⁺ promotes intense coaggregation. Combinations of DMSO and metal ions show a synergistic effect especially for mTau. 2D histograms show the formation of smaller mixed oligomers induced by DMSO compared to the metal ions. B. Cross-correlation amplitude before (T₀) and 100 min after (T₁₀₀) addition of SDS to the aggregation assay. Significant reductions of aggregation levels at T₁₀₀ are indicated by the symbols over the error bars, while significant differences between pTau and mTau are indicated by the symbols over the wide bars. C. Cross correlation amplitude at T₁₀₀ normalized against T₀ shows increased SDS-resistance of metal ion induced oligomers compared to DMSO. Measurements were taken from 20 independent samples; each sample was measured four times. Levels of significance are displayed as * = p < 0.05; ** = p < 0.01; ‡ = p < 0.001.