Figure 2

Increased Aβ deposition in AAV1-mIL-4 expressing TgCRND8 mice. A-D. 4 month old TgCRND8 mice were stereotaxically injected in the hippocampus with either AAV1-mIL-4 (C, D) or AAV1-EGFP (A, B) and sacrificed after 6 weeks (n = 5-6/group). Representative brain sections stained with 33.1.1 antibody (pan Aβ 1–16) depict increased Aβ deposition in mIL-4 expressing mice (C, D) compared to controls (A-B) in the immediate vicinity of the injection site. Scale Bar, 150 μm. E. Aβ plaque burden analysis shows a significantly increased amyloid deposition in mIL-4 injected mice compared to control EGFP injected mice (n = 5/group). (*p < 0.05, t test). Data represents mean ± sem. F-G. Biochemical analyses of Aβ42 and Aβ40 levels by ELISA show significantly increased SDS (F) and formic acid (G) extractable Aβ levels in mIL-4 injected mice compared to controls (n = 5/group). (*p < 0.05, t test). Data represents mean ± sem.