Figure 4
From: Hippocampal expression of murine IL-4 results in exacerbation of amyloid deposition
mIL-4 does not affect human APP transgene expression or CTF production. A-B. Representative anti CT20 immunoblot showing no significant changes in APP, CTFα and CTFβ levels in AAV1-mIL-4 expressing TgCRND8 compared to age-matched controls (A). Intensity analysis (mean ± sem) of anti CT20 immunoreactive APP and CTFα levels was normalized to β-actin in TgCRND8 mice cohort (B). (n = 5/group; p > 0.05, t test). C-D. Representative immunoblot showing no significant changes in prion protein levels in AAV1- mIL-4 TgCRND8 compared to age-matched controls (C). Intensity analysis of immunoreactive prion protein levels (mean ± sem) was normalized to β-actin (D). (n = 5/group; p > 0.05, t test). E-F. Representative anti CT20 immunoblot showing no significant changes in APP and CTFα in 5 month old AAV1-mIL-4 expressing wild type B6/C3H mice compared to age-matched controls (E). Intensity analysis of anti CT20 immunoreactive APP and CTFα levels (mean ± sem) was normalized to β-actin in wild type mice cohort (F). (n = 5/group; p > 0.05, t test).