Figure 5

Characterization of rAAV2/1-mIL-4 treated primary wild type mouse neuroglial culture. A-D. rAAV2/1-mIL-4 or rAAV2/1-mIFN-γ (2x10⁸ viral genomes) was used to transduce primary mouse neuroglial cultures for 60 hrs in chamber slides. Upregulation of phosphorylated STAT6 (568 nm; red fluorescence) could be seen in mIL-4 expressing cultures (C) but not in mIFN-γ expressing cultures (B) or untreated culture containing no viruses (A). MAP2 (488 nm; green) and DAPI (350 nm, blue) were used to depict neuronal processes and nucleus respectively. mIL-4 and mIFN-γ expression in these cultures was verified by ELISA from media collected from respective cultures (D). Magnification, 400x. (*p < 0.05, t test). E. Expression of mouse endogenous APP, BACE1, IDE and neprilysin levels are unchanged in rAAV2/1-mIL-4 expressing primary mixed neuroglial cultures compared to untreated control cultures using real time Q-PCR. mIL-4 expression augments scavenger receptors and cd11b levels. Data, expressed as relative levels of mRNA expression, represents averaged fold change values obtained from mIL-4 expressing mice, relative to averaged values obtained from EGFP expressing mice. Data for mIL-4 RNA is plotted on the left x-axis while the rest of the data are plotted on the right x-axis. Data is representative of two independent experiments (t test, *p < 0.05). Data represents mean ± sem.