Skip to main content
Figure 2 | Molecular Neurodegeneration

Figure 2

From: Nurr1 regulates Top IIβ and functions in axon genesis of mesencephalic dopaminergic neurons

Figure 2

NURR1 is necessary for the maintenance of TOP IIβ expression in cell cultures. A. Knock-down NURR1 expression level in N2a cells by stealth siRNA down-regulates TOP IIβ protein level. Nucleoprotein of the cells was extracted 3 days after transfection. B. Statistic analysis of the protein levels. Data obtained for NURR1 and TOP IIβ expression levels were normalized to LAMIN B expression level. C. Knock-down NURR1 expression level in N2a cells by stealth siRNA down-regulates Top IIβ mRNA expression. Total RNA of the cells was extracted 48 hours after transfection. We further validated the expression of Nurr1 and Top IIβ mRNA by real-time PCR analysis. Data obtained for Nurr1 and Top IIβ expression levels were normalized to Gapdh expression level. D. Over-expression NURR1 in N2a cells increases TOP IIβ protein level. E. Statistic analysis of the protein levels. F. Over-expression NURR1 in N2a cells increases Top IIβ mRNA expression level. Expression levels expressed relative to a negative control (random sequence). Each bar represents the average of three individual wells, and samples were triplicate. Error bars indicated the standard error for each group. **P <0.01 vs. negative control.

Back to article page