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Table 2 Validation of proteolytic digestion

From: Mass spectrometry quantification of clusterin in the human brain

  Recombinant clusterin/QconCAT clusterin (pmol/pmol)
  Mixed Measured
ASSIIDELFQDR (Q1) 1.0 1.2 ± 0.1
  2.0 2.4 ± 0.2
ELDESLQVAER (Q4) 1.0 1.3 ± 0.2
  2.0 1.9 ± 0.3
VTTVASHTSDSDVPSGVTEVVVK (Q5) 1.0 1.2 ± 0.2
  2.0 2.4 ± 0.3
consensus 1.50 (100%) 1.73 (116%)
  1. The measurements were performed for three experimental replicates by monitoring three transitions per individual peptide and presented as mean ± SD. For consensus, the mean measured ratio is presented as a percentile of mean mixed ratio.