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Table 2 Validation of proteolytic digestion

From: Mass spectrometry quantification of clusterin in the human brain

 

Recombinant clusterin/QconCAT clusterin (pmol/pmol)

 

Mixed

Measured

ASSIIDELFQDR (Q1)

1.0

1.2 ± 0.1

 

2.0

2.4 ± 0.2

ELDESLQVAER (Q4)

1.0

1.3 ± 0.2

 

2.0

1.9 ± 0.3

VTTVASHTSDSDVPSGVTEVVVK (Q5)

1.0

1.2 ± 0.2

 

2.0

2.4 ± 0.3

consensus

1.50 (100%)

1.73 (116%)

  1. The measurements were performed for three experimental replicates by monitoring three transitions per individual peptide and presented as mean ± SD. For consensus, the mean measured ratio is presented as a percentile of mean mixed ratio.
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Molecular Neurodegeneration

ISSN: 1750-1326