Mass spectrometry quantification of clusterin in the human brain

Table 3 Quantification of clusterin in the human brain tissues

	Clusterin, pmol/mg tissue protein
	Frontal cortex		Temporal cortex
	Control	Severe AD	Control	Severe AD
ASSIIDELFQDR (Q₁)	26.0 ± 4.2	39.6 ± 9.0	29.5 ± 7.4	24.2 ± 8.5
VTTVASHTSDSDVPSGVTEVVVK (Q₅)	24.8 ± 4.7	38.6 ± 9.3	27.8 ± 7.9	29.3 ± 7.7
consensus	25.4 ± 4.4	39.1 ± 9.1	29.0 ± 7.9	28.0 ± 8.4

Each control and severe AD group includes six human donors. The concentration was calculated for three experimental replicates by monitoring three transitions per individual peptide and presented as mean ± SD. The monitored transitions are summarized in Table 1. The reported phosphorylated Thr and Ser residues [10] are underlined. For the consensus, the data for both peptides were combined and presented as mean ± SD. Clusterin concentrations in frontal cortex were significantly different (P < 0.01) using a t test comparison for the control and severe AD groups, whereas clusterin concentrations in temporal cortex were not significantly different.

ISSN: 1750-1326