Figure 1
From: Exosomal cell-to-cell transmission of alpha synuclein oligomers
Extracellular αSyn oligomers are associated with exosomes: (A) Exosomal fractions from human H4 cells transfected with αsyn complementation pair S1 and S2 contain high amounts of αsyn oligomers, analyzed with a luciferase assay. Signal corresponds to 1.25x100mm dishes. n = 6, unpaired t test with Welch's correction, ***p < 0.001 (B) αsyn ELISA analysis from exosomal fractions from human H4 cells transfected with S1/S2, wt αsyn or Mock. Signal corresponds to 1x100 mm dish. n = 4, unpaired t test with Welch's correction, ***p < 0.001 (C) Exosomal fractions from primary neurons infected with αsyn complementation pair S1 and S2 contain high amounts of αsyn oligomers, analyzed with a luciferase assay, signal corresponds to 1x60 mm dish, n = 4, unpaired t test with Welch's correction, ***p < 0.001 (D) αsyn ELISA analysis from exosomal fractions from primary neurons infected with AAV expressing GFP, αsyn -ires-GFP, V1S/SV2 and S1/S2. Values correspond to 1x60 mm dish, n = 4, Tukey's Multiple Comparison Test, ***p < 0.001.