Figure 5

Exosomes need to be intact to be internalized: (A) DiD labeled exosomes containing αsyn with or without prior sonifications were added to H4 cells transfected with Venus-YFP and incubated for 3 days. Scale bar = 20 μm (B) Exosomal fractions derived from H4 cells transfected with αsyn-luciferase were applied to naive H4 cells after sonication or without sonication and incubated for 3 days. Uptake of αsyn oligomers into recipient neurons was measured performing a luciferase assay on recipient cells. n = 5, one-sample t-test, *p < 0.05. (C) DiD labeled exosomes containing αsyn with or without prior sonifications were added to primary cortical neurons infected with AAV-Venus-YFP incubated for 3 days. Scale bar = 20 μm (D) Co-localization of exosomal marker flotillin (green) and DiD labeled exosomes (red) after addition of exosomes containing αsyn to naïve H4 cells. Scale bar = 20 μm.