Figure 1From: ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBITVimentin modifies mutant Htt aggregation. A. Representative confocal images show distribution of normal (16Q) and pathogenic (60Q and 150Q) tNHtt (green) and RFP or RFP-vimentin (red) in inducible tNHtt-polyQ-EGFP Neuro2a cells. Note the cages formed by vimentin in 60Q and 150Q Neuro2a cells. Nuclei were stained with DAPI (blue). Scale bar, 5 μm. B. RFP-vimentin expression increased and vimentin knock-down reduced polyQ aggregation and levels of total mutant Htt in 150Q Neuro2a cells as compared to the control. C. The effect of RFP-vimentin on Htt levels is polyQ length-dependent. While tNHtt-60Q-EGFP and tNHtt-150Q-EGFP accumulated as the insoluble forms at the gel top, tNHtt-16Q-EGFP levels remained unchanged upon RFP-vimentin transfection.Back to article page