Figure 4From: ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBITVimentin phosphorylation affects IRBIT subcellular distribution. Effect of ROCK and UPS inhibitors. Representative confocal images show subcellular distribution of IRBIT in presence of RFP or tested RFP-vimentin forms. Neuro2a cells were transfected and treated as indicated for 24 hrs. Cells were fixed, permeabilized and immunostained for enogenous IRBIT (green). A. Control untreated cells (DMSO, 1/1000). B. Cells treated with 20 μM Y-27632. C. Cells treated with 5 μM MG132. Fluorescence of RFP (red) and DAPI (blue) are also shown. Scale bar, 5 μm.Back to article page