Vimentin phosphorylation affects IRBIT subcellular distribution. Effect of ROCK and UPS inhibitors. Representative confocal images show subcellular distribution of IRBIT in presence of RFP or tested RFP-vimentin forms. Neuro2a cells were transfected and treated as indicated for 24 hrs. Cells were fixed, permeabilized and immunostained for enogenous IRBIT (green). A. Control untreated cells (DMSO, 1/1000). B. Cells treated with 20 μM Y-27632. C. Cells treated with 5 μM MG132. Fluorescence of RFP (red) and DAPI (blue) are also shown. Scale bar, 5 μm.