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Figure 5 | Molecular Neurodegeneration

Figure 5

From: ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBIT

Figure 5

IRBIT modifies inclusion formation in 150Q Neuro2a cells. A. 48 hrs after transfection of control or IRBIT siRNA and RFP or tested RFP-vimentin forms, cells were induced for 24 hrs, fixed, and analyzed by ArrayScan. *p = 0.028, **p = 0.0008, ***p = 0.00005, ****p = 0.00004, ˆp = 0.0018, ˆˆp = 0.0021, ˆˆˆp = 0.00034, #p = 0.0007, ##p = 0.0004, ###p = 0.00002, #167;p = 0.016, #167;#167;p = 0.0001. B. Images generated and analyzed by ArrayScan representing each experimental condition in A. Green, tNHtt-150Q-EGFP; blue, Hoechst 33258. Magnification, 20x. C. Cells were co-transfected with mock or flag-IRBIT and RFP or tested RFP-vimentin forms and induced for 24 hrs before analyzed by ArrayScan. *p = 0.042, **p = 0.0002, ***p = 0.049, ˆp = 0.0002, ˆˆp = 0.0099, ˆˆˆp = 0.00013, #p = 0.00005, ##p = 0.015, ###p = 0.00009, ####p = 0.0008, #####p = 0.0005, ######p = 0.0092, #167;p = 0.0026, #167;#167;p = 0.0016. D. Images generated and analyzed by ArrayScan representing each experimental condition in C. Green, tNHtt-150Q-EGFP; blue, Hoechst 33258. Magnification, 20x. Bars in A and C represent relative mean values ± s.d. from four independent experiments, with levels of inclusion formation under control conditions normalized to a value of 1.

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