Figure 5From: ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBITIRBIT modifies inclusion formation in 150Q Neuro2a cells. A. 48 hrs after transfection of control or IRBIT siRNA and RFP or tested RFP-vimentin forms, cells were induced for 24 hrs, fixed, and analyzed by ArrayScan. *p = 0.028, **p = 0.0008, ***p = 0.00005, ****p = 0.00004, ˆp = 0.0018, ˆˆp = 0.0021, ˆˆˆp = 0.00034, #p = 0.0007, ##p = 0.0004, ###p = 0.00002, #167;p = 0.016, #167;#167;p = 0.0001. B. Images generated and analyzed by ArrayScan representing each experimental condition in A. Green, tNHtt-150Q-EGFP; blue, Hoechst 33258. Magnification, 20x. C. Cells were co-transfected with mock or flag-IRBIT and RFP or tested RFP-vimentin forms and induced for 24 hrs before analyzed by ArrayScan. *p = 0.042, **p = 0.0002, ***p = 0.049, ˆp = 0.0002, ˆˆp = 0.0099, ˆˆˆp = 0.00013, #p = 0.00005, ##p = 0.015, ###p = 0.00009, ####p = 0.0008, #####p = 0.0005, ######p = 0.0092, #167;p = 0.0026, #167;#167;p = 0.0016. D. Images generated and analyzed by ArrayScan representing each experimental condition in C. Green, tNHtt-150Q-EGFP; blue, Hoechst 33258. Magnification, 20x. Bars in A and C represent relative mean values ± s.d. from four independent experiments, with levels of inclusion formation under control conditions normalized to a value of 1.Back to article page