The atypical sphingoid base 1-deoxySa reduced neuritic branches and outgrowth in primary DA neurons. Primary neuron-glia cultures from rat ventral mesencephalon were plated in 96-well plates and exposed to treatment media alone without BSA (0) or to 1-deoxysphinganine (1-deoxySa) at the concentrations indicated in a complex with BSA (25 μM) for 48 hours prior to assessing number of branches per cell, number of processes, and number of outgrowths per cell as well as cell number using Image Xpress high-content imaging analyses. 1-DeoxySa was the only one of the sphingoid bases tested that reduced neurite outgrowth and branching. All values represent group means +/− SEM, n = 3–4. One-way ANOVA with Tukey’s post-hoc’ * denotes difference from treatment media alone at p < 0.05, and ** denotes p < 0.01. N.S. denotes not significant.