Figure 9From: Ceramide sphingolipid signaling mediates Tumor Necrosis Factor (TNF)-dependent toxicity via caspase signaling in dopaminergic neuronsAtypical sphingoid bases generated in response to TNF exert direct cytotoxic effects on neurally differentiated MN9D dopaminergic (DA) cells. MN9D cells were plated in 96-well plates, and were neurally differentiated as described under Methods and exposed to treatement media alone (without BSA) or to various doses of 1-deoxysphinganine (1-deoxySa), 1-desoxymethylsphinganine (1-desoxyMeSa), or 1-desoxymethylsphingosine (1-desoxyMeSo) at the concentrations indicated in a complex with BSA (25 μM) for 48 hours. Cell viability was measured by the MTS assay described under Methods. All values represent group means +/− SEM, n = 3–4. One-way ANOVA with Tukey’s post-hoc, * denotes difference from treatment media alone (without BSA) at p < 0.05, *** denotes difference from media alone at p < 0.001.Back to article page