Figure 3

Phospho-tau-positive inclusions in conditional Atg7 -deficient mice. (a-c) Phospho-tau-positive inclusions in cortical neurons of CamK-Atg7 cKO mice. a, AT8, an antibody against phospho-tau at Ser202/Thr205 residues (red, arrows), stained p62-positive inclusions (green) in cortical neurons, with slight background signals (asterisks) in cell nucleus, of CamK-Atg7 cKO mice. Bar, 10 μm. b, AT100, an antibody against phospho-tau at Ser212/Thr214 residues (red, arrows), stained p62-positive inclusions (green) in cortical neurons, with slight background signals (asterisks) in cell nucleus, of CamK-Atg7 cKO mice. Bar, 10 μm. c, TG3, an antibody against phospho-tau at Thr231/Ser235 residues (red, arrows), stained p62-positive inclusions (green) in cortical neurons of CamK-Atg7 cKO mice. Bar, 10 μm. (d) Immunoelectron microscopic analysis of phospho-tau-positive inclusion in CamK-Atg7 cKO mice. Immunoelectron microscopic analysis of inclusions using the phospho-tau-specific antibody TG3 and horseradish peroxidase staining (dark speckles; contrast to Figure. 1f). At right is a high magnification image of the inset (dashed square in left). Bars, 2 μm (left) and 500 nm (right). (e) Phospho-tau levels are elevated in forebrain extracts of CamK-Atg7 cKO mouse. Western blotting reveals that AT8-, AT100- or TG3-positive phospho-tau is significantly increased in both 0.5% Triton X-100-soluble and -insoluble fractions of CamK-Atg7 cKO brain tissue extracts. AT270- or PHF1-positive phospho-tau and total tau [Tau1] were not changed. n = 5. Two independent samples are presented for each genotype, as labelled at bottom.