Figure 3

Nmnat by itself does not protect dopaminergic neurons from MPP⁺ toxicity. (A) Diagram of the lentiviral constructs used to transduce WT dissociated dopaminergic neurons. (B) Western blot of cell lysates from transduced primary midbrain cultures illustrates that all the transduced transgenes exhibit similar levels of expression. (C) Similar transduction efficiencies of the different lentiviruses were confirmed by quantifying the number of TH+ and GFP+ cells following transduction of dopaminergic cultures. (D) Quantification of TH+ cell bodies and (E) TH+ neurites show that only Wld^S-transduced cultures protected neurites against MPP⁺. Data are normalized to control cultures and denote the mean ± SEM of representative determinations made in three separate cultures. *p < 0.001.