Figure 4From: Hyperpolarization-activated cyclic nucleotide gated channels: a potential molecular link between epileptic seizures and Aβ generation in Alzheimer’s diseaseFunctional deficits in the HCN1 channel facilitate Aβ generation. (A) Quantification of endogenous murine Aβ40 and Aβ42 in the cortex of 4-month-old HCN1+/+, HCN1+/-, and HCN1-/- mice by using the sELISA system (left panels). Statistical analysis was performed using the two-tailed Mann–Whitney U-test (average ± SEM, n = 5, **p < 0.01). (B) Quantification of Aβ40 and Aβ42 secreted into the culture medium by N2a cells by using the sELISA system. FLAG-APP or FLAG-APP and HCN1 were transiently overexpressed in the cells with (+) or without (−) 10 μM ZD7288 (left panels). Statistical analysis was performed using one-way analysis of variance followed by Tukey’s multiple comparison test (average ± SEM, n = 4, ***p < 0.001). Expression levels of APP and HCN1 was confirmed by immunoblotting (right panels in Aand B).Back to article page