Figure 4
From: Endogenous TDP-43, but not FUS, contributes to stress granule assembly via G3BP
Endogenous FUS does not function in SG assembly in response to sodium arsenite. (A - E) SG were assessed in HeLa cells transfected with control or FUS siRNA for 72 hr and subsequently treated with SA. (A & B) Quantification of individual SG size and number of SG per cell. (C & D) Western blots and quantification of protein levels demonstrating that FUS does not regulate G3BP, TIA-1 or TDP-43, in HeLa cells (left panel) nor in SK-N-SH cells (right panel). Actin serves as a loading control. The means of 3 independent experiments ± SEM are plotted. * p < 0.05