Figure 5
From: Endogenous TDP-43, but not FUS, contributes to stress granule assembly via G3BP
Neuronal-like cells have increased vulnerability to blocked SG assembly. (A & B) HeLa and (C & D) SK-N-SH cells treated with the indicated siRNAs for 48 hr and then exposed to SA for 30 min. (A & C) Western blots confirming siRNA transfections in (A) HeLa and (D) SK-N-SH cells. Actin serves as a loading control. (B & D) Cell death determined by trypan blue exclusion at the indicated times following SA treatment in (B) HeLa and (D) SK-N-SH cells. The mean percentage of cell death is presented ± SEM of 3 independents experiments. * p < 0.05