Figure 6
From: Endogenous TDP-43, but not FUS, contributes to stress granule assembly via G3BP
HeLa cells have increased cell sensitivity to sodium arsenite in the presence of modestly elevated GFP-TIA-1 expression. (A–C) HeLa (left panels) and SK-N-SH (right panels) cells were transfected with GFP or GFP-TIA-1 for 48 hr. (A) Representative images of HeLa (left panels) and SK-N-SH (right panels) cells transfected with GFP or GFP-TIA-1 for 48 hr and then stained with TO-PRO3 to label nuclei. (B) Western blot confirming TIA-1 expression. Asterisk (*) indicates non-specific bands from TIA-1 antibody. (C) Cell viability was assessed by Annexin V labelling in HeLa and SK-N-SH. The means of 3 independent experiments ± SEM are plotted. * p<0.05