FPR1 and FPRL1 are co-localised with RAGE in primary rat glial cells. Astrocytes and microglial cells were fixed and labelled with anti-FPR1 (A) or anti-FPRL1 (B) and anti-RAGE antibodies. Localisation of FPR1, FPRL1 and RAGE was examined by double fluorescence microscopy. Bisbenzimide was used for nuclear counter-staining (blue). The figures show representative results from one of three independent experiments, each performed in duplicate. Scale bar: 20 μm. The right columns showed resulting scatter plot of intensities across the two channels. The Pearson correlation coefficient rp and Spearman correlation coefficient rs are indicated on the scatter plots. For FPR1 (green channel) and RAGE (red channel) (A), low co-localisation and for FPRL1 (green channel) and RAGE (red channel) (B) good co-localisation.