Figure 3
In vivo Aß 1–42 aggregation . Fluorescence micrographs of adult C. elegans heads. A ThT positive Aß aggregates are absent in transgenic control strain CL2122 (top), and marked with arrow heads in CL2120, expressing Aß3-42 (middle), and GMC101, expressing Aß1-42 (bottom). B X-34 positive Aß1-42 aggregates are seen (arrow head), CL2122 (top) and GMC101 (bottom). Scale bar = 25 μm. C. Size-excluded soluble proteins from Aß1-42 expressing GMC101. Shown is the absorbance at 280 nm (A280) of eluted material against elution volume. Fractions (0.75 ml) were collected from 5.25 ml onwards (marked by arrow). D Immuno-blot analysis of size-excluded fractions (F2-18) resolved via 4-12% BisTris SDS PAGE and detected with 6E10. Shown are starting material extracts from strain GMC101 (Aß1-42) and control strain CL2122. Aß1-42 elutes predominantly in fractions F4-F5. A longer exposure (below) shows Aß also elutes in fractions consistent with lower-order oligomers (i.e. F11-F13).