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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Utility of an improved model of amyloid-beta (Aβ1-42) toxicity in Caenorhabditis elegansfor drug screening for Alzheimer’s disease

Figure 5

PBT2 protects against Aß 1–42 induced toxicity . Paralysis associated with Aß1-42 expression is protected by PBT2. Plotted are the proportions of individuals not paralysed with upper and lower 95% confidence intervals. A PBT2 at 10 μg/ml was found to be most effective when cultures were exposed to compound for 24 prior to the initiation of the assay. B PBT2 effects are very rapid, such that immediate exposure to 10 μg/ml PBT2 suppressed Aß-induced paralysis. Control strain CL2122 (n = 74), Aß1-42 strain GMC101 (n = 73), GMC101 strain + PBT2 (n = 74), where n = number of individuals assayed and *** p < 0.001. Plot shown is representative of 3 experiments. C Exposure to 10 μg/ml PBT2 for 24 h did not significantly alter total Aß levels. Shown is mean ± SD quantitation via densitrometry of n = 3 immuno-blot analyses. D Exposure to PBT2 does not alter DAF-16 localization. Shown are representative epifluorescence micrographs of DAF-16: GFP cytoplasmic localization in Control and PBT2 (10 μg/ml for 24 h) treated populations in contrast to nuclear localization in samples heat-shocked for 2 h at 35°C.

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