Method | Genetic background | Brain region | Cell or neuron number | Age of animals and transgene(s) harbored | Reference |
---|---|---|---|---|---|
Isotropic Fractionator | TgCRND8 (C3H/He-C57BL/6) | Whole brain | 100.10 ± 7.90 x 106 | 6 months; K670M/N671L/V717F APP | Present study |
Dutch (C57BL/6J) | |||||
Isotropic Fractionator | Swiss Webster | Whole brain | 108.90 ± 16.25 x 106 | 2-5 months | |
Isotropic Fractionator | TgCRND8 (C3H/He-C57BL/6) | Cerebellum | 43.92 ± 2.78 x 106 | 6 months; K670M/N671L/V717F APP | Present study |
Dutch (C57BL/6J) | |||||
Isotropic Fractionator | Swiss Webster | Cerebellum | 49.17 ± 5.32 x 106 | 2-5 months | |
Isotropic Fractionator/Flow Cytometry | C57Bl/6J | Cerebellum | 44.03 ± 0.42 x 106 | 60-100 days | |
Isotropic Fractionator | TgCRND8 (C3H/He-C57BL/6) | Hippocampus | 31.8% decrease in neurons | 6 months; K670M/N671L/V717F APP | Present Study |
Isotropic Fractionator | Dutch (C57BL/6J) | Hippocampus | No decrease in neurons | 18 month Dutch E693Q APP mice | Present study |
Stereology | C57BL/6 | CA1 | ~25% decrease in neurons | 14-18 month-old TgAPP23 (K670M/N671L) mice | |
Stereology | APP/ PS1M146L (CBA - 12.5% × C57Bl6 - 87.5%) | CA1-CA3 | ~30% decrease in neurons | 17 month APP (751SL) and presenilin 1 (PS1M146L) mice | |
Controls (C57BL/6) | |||||
Stereology | (C57BL/6 50%-CBA 25%–129SV 25%) | CA1/2 | ~50% decrease in neurons | 10 month APPSLPS1KI (APP K670N/M671L, and V717I; PS1 M233T/L235P knock-in) | |
Stereology | C57BL/6J | CA1 | 33% decrease in neurons | 6 month APP/PS1KI | |
(APP K670N/M671L, and V717I; PS1 M233T/L235P knock-in) |