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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Defective lysosomal proteolysis and axonal transport are early pathogenic events that worsen with age leading to increased APP metabolism and synaptic Abeta in transgenic APP/PS1 hippocampus

Figure 5

In vitro inhibition of either lysosomal and/or proteasomal activities induced the amyloidogenic APP processing in N2a-APPswe cultures. A) The inhibition of proteasomal/lysosomal (MG132) or lysosomal (Chloroquine) activities produced the accumulation of both ubiquitinated proteins (upper panel) and LC3-II autophagosomal marker (middle panel). Graphs showed the quantitative data of three different experiments using three different N2aAPPswe cultures. B) Using the same protein samples as in (A), we analyzed the effect of either inhibitor on the APP-CTF processing (upper western) and intracellular Abeta accumulation (lower western). As also shown quantitatively in graphs, either treatment produced the increase in the APP processing and the intracellular Abeta accumulation. C) In parallel, the expression of BACE-1 and PS1-ctf was also tested. As showed quantitatively, after 24h of treatment, both BACE-1 and PS1-cft were significantly accumulated in N2aAPPswe cells.

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Molecular Neurodegeneration

ISSN: 1750-1326