Figure 7

Chronic daily exposure to EVP-0015962 in Tg2576 mice reduces Aβ peptide levels. A-F. TBS soluble (A, C, E) and formic acid extractable (B, D, F) Aβ peptide levels determined by ELISA from the brains of Tg2576 mice after 50 weeks of treatment with EVP-0015962 for Aβ₄₂ (A-B), Aβ₃₈ (C-D), and Aβ_Total (E-F). N = 5 mice per group. Differences from the control diet group: *p < 0.05, **p < 0.01, ***p < 0.001 (1-way ANOVA, post-hoc Newman-Keuls test). G-H. Aβ₄₂ peptide levels measured at the beginning of treatment and after 11, 28, and 50 weeks of treatment with EVP-0015962, showing the time course of accumulation of Aβ₄₂. G. TBS soluble Aβ₄₂. H. Insoluble Aβ₄₂. At 11 and 28 weeks, insoluble Aβ₄₂ was extracted with only formic acid. At 0 and 50 weeks, the 4-step procedure was used, and the insoluble fraction is the sum of the Aβ₄₂ extracted with Triton®-X, SDS, and formic acid. N = 4–6 mice per group and time point. Graphs illustrate mean ± SEM.