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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody

Figure 2

MOAB-2 does not detect APP in cell culture media and lysates or cortical brain extracts from 5xFAD mice. (A) Western-blot analysis of 5 μg or 15 μg cell lysates from HEK-APPSwe/BACE1 cells, probed with antibodies against C-terminus of APP (CT1565), N-terminus of APP (2211), Aβ (6E10, MOAB-2) or β-Actin (loading control). (B) Western analysis of 25 μg total protein from detergent-extracted 5xFAD mouse cortex probed with 6E10 or MOAB-2 and β-Actin for loading control. (C) DAB staining with 6E10 or MOAB-2 in 1- and 3-month old 5xFAD mice. Box insert: single neuron staining (Coronal sections, top = 10x scale, bar 100 μm; bottom = 100x, scale bar 20 μm). The dilution of MOAB-2 for Figures 2A,B and C is 1:5000 from a stock of 0.5 mg/ml. (See "Dot- and Western-blot Analyses" in Methods for other antibody concentrations.).

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