Figure 3
From: Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody
MOAB-2 detection of intraneuronal Aβ but not intraneuronal APP in 5xFAD brain tissue. Immunofluorescent detection of Aβ and APP in the cortex of: (A) 1-month old 5xFAD mice using: MOAB-2, Aβ42- or Aβ40-specific antibodies; (B) 1-month old 5xFAD mice with MOAB-2 and antibodies against the C-terminus (Cter) (CT695) or N-terminus (Nter) (2211) of APP; and (C) 4-month old 5xFAD/BACE-/- with 6E10 or MOAB-2, plus antibodies CT695 and 22C11. (Coronal sections, representative confocal images at 100x). Antibody concentrations: MOAB-2 (1:1000), anti-Aβ40, (1:1700), anti-Aβ42 (1:200), 22C11 (1:40), CT695 (1:500), 6E10 (1:1000).