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Figure 2 | Molecular Neurodegeneration

Figure 2

From: FE65 as a link between VLDLR and APP to regulate their trafficking and processing

Figure 2

FE65 co-localizes with VLDLR in primary hippocampal neurons. A. After 3 days in culture, primary hippocampal neurons were fixed and immunostained for 5F3 (recognizing VLDLR) and anti-FE65. Antibodies were detected with Alexa Flour 488 anti-mouse antibody (in green for VLDLR) and Alexa Fluor 555 anti-rabbit antibody (in red for FE65), imaged with a confocal laser-scanning microscope (63X). Co-localization of VLDLR and FE65 appears yellow as vesicular staining in the right panel. B. After 14 days in culture, primary hippocampal neurons were fixed and immunostained for 5F3 (recognizing VLDLR) and anti-FE65. Antibodies were detected with Alexa Flour 488 anti-mouse antibody (in green for VLDLR) and Alexa Fluor 555 anti-rabbit antibody (in red for FE65), observed with a confocal laser-scanning microscope (63X). Co-localization of VLDLR and FE65 appears yellow as vesicular staining in the right panel, consistent with Figure 2A.

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