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Figure 6 | Molecular Neurodegeneration

Figure 6

From: FE65 as a link between VLDLR and APP to regulate their trafficking and processing

Figure 6

VLDLR interacts with APP and affects the processing of both proteins. A. Mouse brain lysates (100 ug) were immunoprecipitated with 5F3 (VLDLR antibody) or IgG antibody and probed with the C1/6.1 antibody for APP. B. Mouse brain lysates (100 ug) were immunoprecipitated with C1/6.1 (APP antibody) or IgG antibody and probed with 5F3 antibody for VLDLR. C. COS7 cells were transfected with VLDLR-myc and vector (lanes 1, 3, 5) or VLDLR-myc and APP-HA (lanes 2, 4, 6). Secreted VLDLR was measured in conditioned media with 5F3 antibody, and total levels of VLDLR and APP were measured in cell lysates with anti-myc antibody and C1/6.1, respectively. D. COS7 cells were transfected with APP and vector (lanes 1, 3, 5) or APP and VLDLR (lanes 2, 4, 6). Secreted APP was measured in conditioned media with 6E10 antibody, and total levels of APP and APP CTF were measured in cell lysates with C1/6.1. Total levels of VLDLR were measured in cell lysates with anti-myc antibody.

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