Figure 7
From: FE65 as a link between VLDLR and APP to regulate their trafficking and processing
The interaction between APP and VLDLR regulates the cell surface levels of both proteins. A. COS7 cells were transfected with VLDLR and vector (lanes 1, 3) or VLDLR and APP (lanes 2, 4). Cell surface proteins were biotin-labeled, isolated with avidin-beads, and immunoblotted for VLDLR. APP increased surface levels of VLDLR (upper blot). B. COS7 cells were transfected with APP and vector (lanes 1, 3) or APP and VLDLR (lanes 2, 4). Cell surface proteins were biotin-labeled, isolated with avidin-beads, and immunoblotted for APP. VLDLR increased surface levels of APP (upper blot). C. Cultured hippocampal neurons (DIV14) were transfected with GFP, APP, and empty vector (upper panel) or GFP, APP, and VLDLR (lower panel). Surface APP was measured with an anti-APP antibody by immunofluorescence of live cells. D. Quantification of cell surface APP intensity in neuronal processes in (C). The cell surface staining in neuronal processes showed a 24% increase in APP by VLDLR (n = 10, p < 0.05).