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Figure 8 | Molecular Neurodegeneration

Figure 8

From: FE65 as a link between VLDLR and APP to regulate their trafficking and processing

Figure 8

FE65 increased co-precipitation of APP and VLDLR. A. COS7 cells were transfected with APP-v5, empty vector, and VLDLR-HA or APP-v5, FE65-GFP, and VLDLR-HA. Cell lysates were immunoprecipitated with anti-HA antibody (for VLDLR) and probed with anti-V5 (for APP). Immunoblots of cell lysates showed similar levels of APP and VLDLR. B. COS7 cells were transfected with APP, empty vector, and VLDLR or APP, FE65, and VLDLR. Cell lysates were immunoprecipitated with anti-V5 antibody (for APP) and probed with anti-HA (for VLDLR). Immunoblots of cell lysates showed similar levels of APP and VLDLR. C. COS7 cells were transfected with APP, VLDLR and either full length FE65-GFP or FE65-PTB2 domain-GFP. Cell lysates were immunoblotted with anti- GFP antibody (for FE65 constructs), VLDLR, APP and β-actin was used as the loading control (lower panels). Cell lysates were immunoprecipitated with anti-5F3 (for VLDLR) and probed for anti-22C11 (for APP) (top panel). n = 3 per condition. D. Brain lysates from FE65 knockout mice and wild-type littermates were immunoprecipitated with anti-5F3 and immunobloted for anti-C1/6.1. Immunoblots for total levels of APP (C1/6.1), VLDLR (IIII) and FE65 are in the lower panels.

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