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Figure 1 | Molecular Neurodegeneration

Figure 1

From: Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice

Figure 1

Overexpression of wtVAPB or muVAPB in the spinal cord and brain of the transgenic mice. (A) Schematic diagram showing the transgene construct. This construct has a CAG promoter, which drives the expression of human VAPB gene that is tagged by 3XFLAG (marked as 3XFL) at its N-terminal. The 3X poly A sequences (3XpA) after the VAPB gene terminate the transcription. Therefore, the RFP gene is not expressed. The two triangles represent the loxP sequence that flank the VAPB transgene. (B) VAPB levels from two lines of muVAPB and one line of wtVAPB transgenic mice were determined by Western blot. For the detection of VAPB in the spinal cords, 100 μg protein was loaded in each lane. The proteins were resolved by a 12% SDS-PAGE and detected as described in the materials and methods. For the detection of VAPB in the brains, 30 μg protein was loaded in each lane. Due to its 3XFLAG tag, the molecular weight of the transgenic VAPB (as indicated by *) is slightly more than the endogenous mouse VAPB protein.

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Molecular Neurodegeneration

ISSN: 1750-1326