Figure 3
From: In vivo measurement of apolipoprotein E from the brain interstitial fluid using microdialysis
Analysis of hippocampal ISF apoE3 levels and lipidation. A. The apoE concentration in microdialysis samples collected at flow rates ranging from 0.4 μL/min to 1.6 μL/min was determined by ELISA. Data are presented as mean ± SEM (n=4). A single-phase exponential decay curve (r2=0.93) was used to calculate the estimated mean concentration of apoE3 at zero flow. B. The lipidation of apoE in ISF and CSF samples was analyzed by non-denaturing gel electrophoresis using 4-20% Tris-glycine gradient gels. No apoE was detected in the aCSF used for microdialysis. The samples were run on the same gel; however the CSF panel is from a shorter exposure than the ISF panel for clarity.