Figure 1

Characterization and quantification of 6-OHDA induced lesions. A) Apomorphine-induced turning behavior revealed two classes of 6-OHDA lesioned animals, those with reduced turning behavior (incomplete lesion) and those with intense turning behavior (complete lesion). Sham animals showed no turning behavior. B) Representative photomicrographs of brain slices stained for TH. Compared to sham group, both incomplete and complete lesion groups display marked reduction of TH staining in areas A9 and A10 of the lesioned hemisphere (*). Scale bar = 1 mm. C) Quantification of lesion extent in area A9 shows a ~70% reduction of TH staining in the incomplete lesion group and a ~97% reduction in the complete lesion group. D) Quantification of lesion extent in area A10 shows a ~40% reduction of TH staining in the incomplete lesion group and a ~60% reduction in the complete lesion group. Sham n = 19; incomplete lesion n = 13; complete lesion n = 28. For histological analysis sham n = 5; incomplete lesion n = 4; complete lesion n = 5. Data presented as mean ± SEM. ** p < 0.01, *** p < 0.001.