Figure 1

The levels of the presynaptic gutamatergic transporter VGlut in hippocampal neurons of 4-month-old apoE3 and apoE4 mice. (A) VGlut1 immunohistochemistry. Representative images (X20 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM: n=20 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis as described in Materials and Methods and is shown on the right. *p<0.001 (for comparison of the results of the apoE4 and apoE3 mice). Scale = 120 μ. (B) VGlut immunoblot. Representative immunoblots of homogenates of whole hippocampi of apoE3 and apoE4 mice are presented on the left together with the GAPDH standard. Quantification of the results (mean ± SEM; n=10 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is depicted on the right. *p<0.001.