Figure 5

Immunohistochemistry of tau phosphorylation and total tau levels in hippocampal neurons of 4-month-old apoE3 and apoE4 mice. (A) Tau phosphorylation immunohistochemistry utilizing AT-8. Representative images (X10 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM; n=20 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis, as described in Materials and Methods, and is shown on the right. *p<0.001. Scale = 350 μ. (B) Total tau levels immunohistochemistry utilizing H150. Representative images (X10 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM; n=20 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis, and is shown on the right. Scale = 350 μ. (C) Tau phosphorylation immunohistochemistry utilizing AT100. Representative images (X10 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM; n=6 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis, and is shown on the right. Scale = 350 μ.