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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Neuron loss in the 5XFAD mouse model of Alzheimer’s disease correlates with intraneuronal Aβ42 accumulation and Caspase-3 activation

Figure 4

Intraneuronal Aβ42 is partially localized within both endosomes and lysosomes in 5XFAD neurons. Parasagittal brain sections of 5XFAD (A, B, E-K) and 5XFAD; BACE1−/− (C, D) brains were co-stained with anti-Aβ42-selective antibody (A-K) and anti-APP (6E10: B, D; Karen [29]: E) or anti-Transferrin receptor (TfR) (F-H) or anti-LAMP1 (I-K) antibody, and DAPI for nuclei (A-K), and imaged by confocal microscopy. All mice were 4 months old, except mice used for LAMP1 immunostaining were 2 months (I-K). Label colors indicate fluorescence color. Note that APP staining appears widely distributed in the soma and cell surface (B, D, E), while Aβ42 labeling is confined to intraneuronal puncta in 5XFAD mice (A, B, E, F, I). Asterisk in E identifies Aβ42-positive plaque (red) above large pyramidal neuron that exhibits intraneuronal Aβ42 puncta (arrow). lntraneuronal Aβ42 signal is absent in 5XFAD; BACE1−/− neurons (C, D), again demonstrating the Aβ42-selectivity of the antibody. In 5XFAD neurons, intraneuronal Aβ42 co-localizes with Transferrin receptor in early endosomes (F-H) and with LAMP1 in late endosomes and early lysosomes (I-K), at least in part. Scale bar in D = 12μm (A, C); = 20 μm (B, D). Scale bar in K = 12 μm (E); = 20 μm (F-K).

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