Exercise attenuates METH-induced up regulation of prooxidative enzymes. Mice were exercised and exposed to METH as described in the legend to Figure 2. Regulatory subunits of NOX, such as phosphorylated-p47 (A; p-p47) and gp91 (B), as well as phosphorylated mitochondrial protein 66Shc (p66Shc) (C) were determined by immunoblotting. Levels of p-47 and gp61 were normalized to β-actin and levels of p66Shc were normalized to total 66Shs. The blots are representative data from three independent experiments and the quantified results (mean ± SEM) are depicted in the form of bar graphs. *As compared to the respective controls at **p < 0.01 or ***p < 0.001. †Data in the exercised group are significantly different as compared to the respective treatment in the sedentary group at ††p < 0.01 or †††p < 0.001.