Figure 6From: LRP1 is critical for the surface distribution and internalization of the NR2B NMDA receptor subtypeLRP1ΔNPxY2 mice show hyperactivity and impaired learning behavior. (A) Activity related analysis in an open field revealed a hyperactivity of LRP1ΔNPxY2 mice (n = 11) compared with WT littermates (n = 15). The diagram represents total distance traveled in open field after 10 min of monitoring ± S.E.M. and demonstrate a significant increase by 13.5% (F(1;24) = 5.060 p < 0.05) for LRP1ΔNPxY2 animals. (B) Learning in the Morris water maze. Test days 1 to 4 display standard spatial learning of the hidden platform task, MANOVA displayed significant effects over time (factor A; F(3;22) = 33.849 p < 0.001) and with respect to genotype (factor B; F(1;24) = 20.980 p < 0.001). Significant post-hoc effects of LRP1ΔNPxY2 mice (n = 11) compared with WT littermates (n = 15) are indicated by *. (C) Memory related behavior based on time mice were searching the platform in a respective quadrant of the maze at the probe trial (day 5) indicated a significant preference (paired t-test *p < 0.05) for the quadrant the platform was located before for WT but not LRP1ΔNPxY2 mice (SWQ southwest quadrant, NEQ northeast quadrant, SEQ southeast quadrant). (D) The sixth test day 24 days after spatial learning indicated both genotypes were able to remember the earlier platform location. Reversal learning abilities are displayed by time to find the platform hidden in a new location at days 7 to 10, MANOVA indicated significant effects over time (factor A; F(3;22) = 11.147 p < 0.001) as well as with respect to genotype (factor B; F(1;24) = 17.736 p < 0.001). Significant post-hoc effects of LRP1Δ NPxY2 mice (n = 11) compared with WT littermates (n = 15) are indicated by *.Back to article page