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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide

Figure 3

The time sequence of engulfment of Aβ 42 by NG2 cells. A. NG2 cell line was incubated with HiLyte Fluor™ 488-labeled Aβ42 (400 nM) for the indicated times, and stained with anti-NG2 antibody. Aβ42 appeared inside the cells after 6 hours of Aβ42 incubation. Scale bars = 5 μm. B-C. Aβ42 engulfed by NG2 cells was quantified with flow cytometry. NG2 cell line (B) and primary NG2 cells (C) were incubated with HiLyte Fluor™ 488-labeled Aβ42 (400 nM) for the indicated times. The amount of Aβ42 inside cells were quantified by measuring the intensity of fluoresce with flow cytometry. Data were presented as mean ± SD; *p<0.05, **p<0.01, compared with cells treated with Aβ42 for 0 hours. The experiments were replicated three times. D. The engulfment of Aβ42 by NG2 cells was concentration-dependent. NG2 cell line was incubated with HiLyte Fluor™ 488-labeled Aβ42 for the indicated concentrations. The amount of Aβ42 inside cells were quantified by measuring the intensity of fluoresce with flow cytometry. Data were presented as mean ± SD; **p<0.01, compared with cells treated without Aβ42. The experiments were replicated three times.

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