Effect of cytochalasin D and nocodazole on Aβ
uptake by NG2 cells. NG2 cell line were treated with cytochalasin D (A), an inhibitor of actin polymerization or nocodazole (B), an agent that disrupts microtubule dynamics for 30 minutes prior to the addition of HiLyte Fluor™ 488-labeled Aβ42 (400 nM) for the indicated times. The amount of Aβ42 inside cells were quantified by measuring the intensity of fluoresce with flow cytometry. Data were presented as mean ± SD; **p<0.01, compared with cells without any treatment (Ct). The experiments were replicated three times.