Figure 5
From: Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide
Localization of Aβ 42 in lysosomes. A. Aβ42 in cellular fraction in NG2 cell line and culture supernatant was measured by immunoblots. The results were quantified and presented as mean ± SD; **p<0.01, denote significant difference from that at 0 hours. The experiment was replicated three times. B. NG2 cell line was incubated with HiLyte Fluor™ 488-labeled Aβ42 (400 nM) for 24 hours, then treated with lysotracker (40 nM) for 30 minutes. Cell nuclei were labeled with DAPI. Aβ42 was co-localized with the signal of lysotracker. Scale bars = 10 μm. C. The mRNA expression of LAMP1 and LAPM2 in NG2 cell line was measured by RT-PCR after treatment with Aβ42 at the indicated times. Data were presented as mean ± SD; **p<0.01, compared with cells treated with Aβ42 for 0 hours. The experiments were replicated three times. D and E. NG2 cell line was exposed to Aβ42 for 6 hours, then treated with leupeptin (20 μM) and pepstatin A (20 μM) for 18 hours. The levels of cellular Aβ42, and LC3, and beclin1 proteins were determined by immunoblotting and normalized to the expression of GAPDH. Data were presented as mean ± SD; *p<0.05, **p<0.01, denote significant difference from the group treated with Aβ42 alone (Ct). The experiments were replicated three times.