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Figure 2 | Molecular Neurodegeneration

Figure 2

From: S-Nitrosylation of parkin as a novel regulator of p53-mediated neuronal cell death in sporadic Parkinson’s disease

Figure 2

Effect of NO on p53 transcription and protein levels in parkin-overexpressing SH-SY5Y cells. A, p53 promoter activity in cells transfected with the control (pcDNA) or Parkin-expression plasmid. The transfected cells were subsequently exposed to 200 μM NO donor GSNO or the control compound GSH. Values are expressed as a percentage of the control (pcDNA-transfected GSH-treated cells). B, Protein levels of parkin, p53 and actin by western blot (left panel) in cells transiently transfected with parkin or pcDNA, and exposed to 200 μM GSNO or GSH. Quantification of p53 levels (right panel) was made relative to actin and expressed in arbitrary units (a.u.). Values are mean + SEM, n = 3; * p < 0.01, ** p < 0.05.

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