Role of p53 in herbicide/fungicide-induced apoptosis in SH-SY5Y cells overexpressing parkin. A, Histological images of cells transiently co-transfected with plasmids for pcDNA or parkin, together with shRNA vectors coexpressing GFP (green). Cells were exposed to a combination of 100 μM paraquat (PQ) and 10 μM maneb (MB) or vehicle for 6 h, fixed, and subjected to TUNEL staining (red). Scale bar: 20 μm. B, Percentage of TUNEL-positive cells among GFP-positive cells under the indicated conditions. Values are mean + SEM of 183–656 cells per condition performed in triplicate experiments; * p < 0.05.